Workshop Report 32

APPENDIX A: WORKSHOP PROGRAMME

Programme Day 1 – Morning

 

10:00-10:30 Registration and welcome coffee
   
10:30-10:40 Welcome and Introduction                                                                            Alan Poole
ECETOC Secretary General
   
Session 1: Understanding ncRNAs
   
10:40-11:00

 

15 minutes +
5 min Q&A

What are ncRNAs?                                                                                           Tim Gant
                                                                                                                             PHE, UK
– Definitions
– Spectrum of ncRNA (sizes, biogenesis)
   
11:05-12:00

15 minutes + 5 min Q&A

 

15 minutes + 5 min Q&A

 

15 minutes + 5 min Q&A

What do they do?                                                                                                           

– Mechanism of action, Functional Relevance                               Gunter Meister
– Importance of negative results                            Regensburg University, Germany

– Expression of macroscopic phenotypes for miRNA-guided             Hervé Seitz
regulation at the molecular level                                                         CNRS, France

 

– Cellular adaptation – relevance for risk assessment              Emma Marczylo
– Physiological interpretation – phenotypic anchoring                            PHE, UK

   
12:00-12:20

15 minutes +
5 min Q&A

Stability and transport                                                                         Kenneth Witwer
                                                                                                  Johns Hopkins University
– Cross-kingdom RNA Effect                                                    School of Medicine, USA
   

 

 

 

Programme Day 1 – Morning (cont’d)

 

12:20-12:40

 

15 minutes + 5 min Q&A

Cause or Consequence?                                                                     Jörg Hackermüller
UFZ, Germany
– What are the links between ncRNA, changes in ncRNA,
expression and pathological phenotype
   
12:40-13:00

 

15 minutes +
5 min Q&A

Exposure effects?                                                                               Nigel Gooderham
                                                                                             Imperial College London, UK
– Relevance of dose level / time / no effect level /
adverse effect level
   
13:00-14:00 Lunch

 

Programme Day 1 – Afternoon

 

Session 2: Understanding possible links between changes
in ncRNA and pathology
   
14:00-14:20

 

 

15 minutes +
5 min Q&A

ncRNA Therapy                                                                                          Achim Aigner
                                                                                               Leipzig University,Germany
– Upcoming medical applications
– What are the current medical applications of ncRNAs?
– How can this help inform toxicology and risk assessment
science?
– Species differences: do animal models inform about
human pathology?
   
14:20-14:40

 

15 minutes +
5 min Q&A

Biomarkers                                                                                   Mohamed Benahmed
                                                                                                                    UNICE, France
– Technical issues around ncRNAs as biomarkers,
(biomonitoring – fluids, and link to pathology –
cells/tissues/organs)
   

 

 

Programme Day 1 – Afternoon (cont’d)

 

Session 3: Technical issues around ncRNA in Risk Assessment
   
14:40-15:00

 

 

15 minutes +

5 min Q&A

Data analysis                                                                                                Frank Slack
BIDMC Cancer Center
Harvard Medical School, USA
– What are the key analysis methods currently employed?
– Is there a need for harmonisation?
– Contamination: is what you are measuring actually what
you think you are measuring?
   
15:00-15:30 Coffee
   
15:30-16:00

 

 

 

20 minutes discussion +
10 min Q&A

Panel Discussion                                                         Moderator: Saskia van der Vies
VU University Medical Centre, NL

Data Interpretation of ncRNAs as biomarkers                                                     Panel:

– There is an explosion of ncRNA data available,                  Tim Gant, PHE CRCE
how is it currently managed?                                                 Reza Rasoulpour, Dow
– What are the gaps/needs?                                             Kerstin Schmidt, BioMath
– What data are relevant for toxicology and risk
assessment?
(What data are relevant for regulations?)

   
16:00-16:20

15 minutes +
5 min Q&A

Risk Assessment considerations for ncRNA in                                           Jan Verhaert
agricultural products/applications                                                   
Monsanto, Belgium
– Current agricultural applications of ncRNA
   
16:20-17:00 Conclusions of Day 1                                                                                      Alan Poole
Details for Networking Cocktail and Day 2                                                   
ECETOC SG
   
17:00 Close
   
18:00-19:00 Networking Cocktail

 

 

Programme Day 2 – Morning

 

09:30-09:40 Welcome, Proceedings for Day 2                                                                  Alan Poole
                                                                                                                                                                        ECETOC, Belgium
   
09:40-10:00 Summary of Day 1                                                                          Saskia van der Vies
VU University Medical Center, NL
   
10:00-11:00 Breakout Session 1:
Role of ncRNAs in toxicology and risk assessment
   
Each breakout group brainstorms both these questions.

Sub-questions are a guide to prompt discussion

Moderator: Tim Gant / Rapporteur: Madeleine Laffont

Moderator: Roland Buesen / Rapporteur: Achim Aigner

Moderator: Helmut Greim / Rapporteur: Alan Poole

 

1.   How to make use of ncRNA technology in toxicology and chemicals risk assessment?

– What is relevant and not relevant?

– What do ncRNAs really tell us?

– How far do ncRNAs really add information to toxicology and risk assessment?

– What role for ncRNAs in toxicology and chemicals risk assessment in the next
5-10 years? (What can they do and what cannot they do?)

 

2.   In light of what we know about ncRNas – should we re-evaluate current toxicology and chemicals risk assessment?

 

– What do changes in ncRNA mean regarding the phenotype?

– What is relevant (cross-kingdom RNA effects)?

– What are the implications for industry/regulations?

– Are the changes in the expression of ncRNA helpful in our
understanding of MoA?

   
11:00-11:30 Coffee

 

Programme Day 2 – Morning (cont’d)

 

11:30-12:30 Plenary:

Moderators share findings from breakout sessions, with room for audience discussion

   
12:30-12:45 Afternoon session explained Madeleine Laffont
ECETOC
   
12:45-13:00 Cefic LRI Programme explained Bruno Hubesch
Cefic-LRI
   
13:00-14:00 Lunch  

 

Programme Day 2 – Afternoon

 

14:00-15:15 Breakout Session 2:
What don’t we know?
Research Priorities for (i) industry (ii) other funders
   
Each breakout group brainstorms the same question. Separate Moderators / Rapporteurs for each group.

30 minutes discussion followed by 45 minutes drafting RfP outline(s).

Moderator: Alan Poole / Rapporteur: Nigel Gooderham

Moderator: Jorg Hackermüller / Rapporteur: Madeleine Laffont

Moderator: Reza Rasoulpour / Rapporteur: Saskia van der Vies

– Which answers are still needed? How can we provide the answers?

– What is currently unsatisfactory about the use of ncRNAs?

– What needs to change?

– What are the priorities?

– Complete outline RfP.

 

Complete RfP template(s)

   
15:15-15:45 Coffee
   
15:45-16:30 Plenary:

Moderators share RfP proposals & outcomes from Session 2. Room for audience participation.

   
16:30 Conclusions and Close                                                                           Helmut Greim
Munich Technical University, Germany